Efficient Microscale Basic Reverse Phase Peptide Fractionation for Global and Targeted Proteomics
نویسندگان
چکیده
منابع مشابه
Cysteinyl Peptide Capture for Shotgun Proteomics: Global Assessment of Chemoselective Fractionation
The complexity of cell and tissue proteomes presents one of the most significant technical challenges in proteomic biomarker discovery. Multidimensional liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based shotgun proteomics can be coupled with selective enrichment of cysteinyl peptides (Cys-peptides) to reduce sample complexity and increase proteome coverage. Here we evaluated the i...
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ژورنال
عنوان ژورنال: Journal of Proteome Research
سال: 2016
ISSN: 1535-3893,1535-3907
DOI: 10.1021/acs.jproteome.6b00102